Angry staff has been filmed throwing medical gloves and shouting "go away" at the Spanish Prime Minister, Mariano Rajoy after his visit to a hospital where a nurse has Ebola Virus Disease, EVD.
Teresa Romero, 44, caught the deadly disease while treating a priest. He had flown to Madrid after becoming infected in West Africa and later died.
Questions have been raised about safety measures at Carlos III hospital where Ms Romero is in a stable but serious condition.
There have been complaints from medical staff that they did not receive the appropriate training.
Rajoy said it was extremely unlikely the disease - which has already killed thousands of people in West Africa - would spread in Spain.
Speaking outside the hospital, the leader said: "Our first priority is Teresa Romero - she is the only person that we know has the illness."
Rajoy said he had set up a committee headed by the deputy prime minister to handle the crisis.
Seven more people have been admitted to the hospital in relation to Romero's case - taking the total number under observation or being treated to 14.